qPCR CNV โ From Relative Quantification to Copy Number
Why this matters
qPCR measures fluorescence during the amplification cycles. Since RT-qPCR includes reverse transcription, it is important to specify both the abbreviation and the template. The ฮฮCq method assumes a stable reference gene, comparable amplification efficiencies, and an appropriate calibrator. For CNV estimation, key factors include diploid controls, assay positioning, DNA quality, and replicates. While qPCR can precisely assess relative copy numbers at target loci, it cannot independently resolve breakpoints, complex rearrangements, tumor purity, or mosaicism.
Interpretation boundary
This article explains the concept and the limits of the evidence. It does not provide diagnostic, testing, or treatment decisions for an individual patient.