ProtocolCore
Suggested Protocols
EVIDENCE-LINKED PROTOCOL LIBRARY
Find protocols in papers, then adapt them to your work
Keep the methods and evidence from each paper visible while changing numbers and conditions or adding steps to create your own protocol.
13
Available
141
Evaluated records PASS
24
Blind holdout PASS
16
Reserve unopened
Choose a protocol field
Only families with explicit sources and validation state are opened. Compare and adapt starters from foundational research through cloning, imaging, and gene or viral delivery.
PRT-NA-FOUNDATION-001
Nucleic Acid Extraction, Purification & QC Foundation
A source-bounded foundation that locks the specimen, DNA or RNA target, and downstream use before one exact extraction profile and orthogonal concentration, purity, integrity, contamination, inhibition, and functional-fit review.
PRT-PROTEIN-PRODUCTION-P1D-001
Recombinant Protein Production Protocol
A source-bounded workflow for one of five routes: BL21/T7, HEK293 transient, CHO transient, CHO stable pool, or CHO clonal producer.
PRT-ANTIBODY-APPLICATION-P1C-001
Manual Antibody Application Protocols
An antibody identity, application-specific validation, control, and reagent-preparation foundation connects to manual Western blot, ELISA, IP/co-IP, ICC/IF, research IHC, and flow cytometry workflows.
PRT-PROTEIN-BOTTOMUP-NORM-001
Bottom-up LC–MS/MS Sample Preparation Protocol
A nine-stage workflow from quantified compatible protein solution through source-specific in-solution digestion, one exact RP peptide cleanup, and facility handoff.
PRT-PROTEIN-INTACT-CHARACTERIZATION-001
Intact Protein Characterization Evidence Record
A research workspace for material lineage and class-specific denaturing/native MS, relative SEC-UV, SEC-MALS, and relative native-gel observations with exact provenance.
PRT-CELL-CULTURE-COMPOSER-001
Cell Culture Lifecycle Protocol
One eight-stage record spanning cell selection and characterization, thawing, seeding, maintenance, passage, harvest, cryopreservation, and banking.
PRT-NGS-NORM-002
NGS Library Preparation Protocols
Manufacturer-guided starters for short-read DNA, stranded mRNA, 16S amplicon, nanopore ligation, and single-cell 3′ sequencing.
PRT-PCR-NORM-002
PCR and Quantitative Amplification Protocols
Source-aware starters that keep conventional PCR, qPCR, RT-PCR, RT-qPCR, and dPCR/ddPCR design, reaction setup, amplification, and quality decisions distinct.
PRT-CRISPR-EXACT-VALIDATION-004
CRISPR / Genome Editing Protocols
Source-bound MVP starters that lock target and reference identity before one SpCas9 knockout, CBE, ABE, HDR, or prime-editing profile and its controls, delivery handoff, and validation contract.
PRT-CLONING-ENZYME-FOUNDATION-001
Cloning & Enzymes Protocols
Source-bound starters that lock an exact profile and revision before restriction-ligation, BsaI-HFv2 Type IIS, or NEBuilder HiFi homology part and junction planning.
PRT-GENE-DELIVERY-TRANSFECTION-001
Gene Delivery / Transfection Protocols
Source-bound starters that lock the cell, payload, reagent, vessel, and source scale before running either suspension HEK293 PEI-MAX antibody-DNA delivery or the exact MDA-MB-231 Lipofectamine 3000 24-well profile.
PRT-IMAGING-ANALYSIS-FOUNDATION-001
Imaging & Analysis Protocols
A fixed-cell fluorescence foundation from a completed specimen handoff through explicit widefield or confocal acquisition, immutable raw artifacts, processing, segmentation, measurements, and replicate-aware summaries.
PRT-VIRAL-DELIVERY-FOUNDATION-001
Viral Delivery Protocols
A research foundation whose exact AAV2 first child separately locks vector, construct, capsid, lot, institutional biosafety disposition reference, native physical-genome titer, target readiness, and outcome lineage.