What this calculator solves

Scale a reviewed reagent recipe by target final amount, batch count, and preparation overage while preserving fixed items and notes.

Scientific review date: 2026-09-16

Formula and variables

scale factor = target / baseline × batch count × (1 + overage/100)

  • • Target amount is the final amount for one batch.
  • • Batch count and overage multiply scalable components.
  • • Fixed components and notes retain their declared behavior.

Worked example

Example: two 500 mL TE batches with 10% overage

Apply a factor of 2.2 to baseline 5 mL Tris-HCl stock and 1 mL EDTA stock.

11 mL Tris-HCl stock + 2.2 mL EDTA stock; 1,100 mL total target final volume

Supported modes

recipe and batch scaling

Scientifically reviewed presets

TE buffer 1× batch recipe (500 mL)

500 mL — 1 M Tris-HCl pH 8.0 stock: 5 mL · 0.5 M EDTA pH 8.0 stock: 1 mL · Add water to the selected final volume.

Why results may differ

  • • The tool does not judge scientific suitability or process equivalence.
  • • It does not generate pH, temperature, sterilization, or mixing instructions.
  • • Edited values are visibly separated from the reviewed preset.

Common questions

How do batch count and overage change component amounts?

Each scalable component is multiplied by batch count and by (1+overage/100).

Which components do not scale?

Items declared fixed and instruction notes are not treated as ordinary scalable numeric components.