Transfection Calculator
Auto-calculate DNA/RNA:reagent ratios for Lipofectamine, PEI, FuGENE, and more. Scale by well plate format.
Invitrogen. Incubate complexes 5 min at RT.
Ratio: 2.5 µL reagent : µg DNA
Area: 9.6 cm² | Volume: 2 mL
TRANSFECTION MIX — 6-well × 1
DNA / well
4 µg
Total DNA
4.00 µg
Reagent / well
10.00 µL
Total reagent
10.00 µL
RECOMMENDED DNA PER WELL (µg)
| Reagent | 6-well | 12-well | 24-well | 48-well | 96-well |
|---|---|---|---|---|---|
| Lipofectamine 2000 | 4 | 1.6 | 0.8 | 0.4 | 0.2 |
| Lipofectamine 3000 | 5 | 2 | 1 | 0.5 | 0.2 |
| FuGENE HD | 2 | 1 | 0.5 | 0.25 | 0.1 |
| PEI (25 kDa, linear) | 4 | 2 | 1 | 0.5 | 0.2 |
| Calcium Phosphate | 10 | 5 | 2 | 1 | 0.5 |
| Electroporation (Neon) | 5 | 2 | 1 | 0.5 | 0.2 |