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Suggested Protocols

탐색형 MVP
전체 프로토콜 분야

CRISPR / Genome Editing

출처가 고정된 CRISPR / Genome Editing 시작 프로토콜

공통 기획부터 SpCas9 knockout, CBE, ABE, HDR, prime editing, 결과 검증까지 exact source profile과 명시적 경계로 구성합니다. 전달 조건은 Gene Delivery handoff에서만 확정합니다.

편집 가능한 시작 프로토콜

7 source-bound 시작안

이 프로토콜은 무엇을 하나요?

공통 identity 계약 뒤 편집 chemistry 하나를 선택하고 source-bound geometry, mode별 control, Gene Delivery 인계, 비의도 산물과 직교 검증을 한 record로 보존합니다.

지원하는 편집 workflow

  • • Common identity, controls & handoffs
  • • SpCas9 nuclease knockout
  • • Cytosine base editing
  • • Adenine base editing
  • • HDR precise editing
  • • Prime editing
  • • Outcome validation

적용 경계

서로 다른 editor·donor·논문의 수치를 혼합하지 않으며 전달 recipe, pooled screen, CRISPRi/a, germline·in vivo·clinical 실행과 보편 PASS 기준은 범위 밖입니다.

PRT-GE-COMMON-001Common identity, controls & handoffs

Genome-editing identity, source, control, delivery, and validation foundation

CRISPR / Genome Editing · GE-COMMON-CONTRACT-1.0

공식 가이드 검토됨
EXACT_SOURCE_PROFILE_REQUIREDGE_COORD_1_REQUIREDNO_CROSS_SOURCE_NUMERIC_MIXINGDELIVERY_HANDOFF_REQUIREDCHEMISTRY_SPECIFIC_OUTCOMESNO_UNIVERSAL_ACCEPTANCE_THRESHOLDRESEARCH_USE_ONLY

실행 전에 표적·source profile·전달 handoff를 확정하세요

이 MVP 시작안은 표적 서열이나 전달 recipe를 만들지 않습니다. 자신의 reference·transcript·edit intent와 exact guide/editor/donor/pegRNA artifact를 입력하고, 실제 Gene Delivery profile과 분석 근거에 맞춰 모든 조건을 확정하세요.

1

1. LOCK MODEL, REFERENCE, AND EDIT INTENT

Record model provenance, assembly and patch, contig, 1-based closed coordinate, strand, HGNC ID, transcript accession/version, allele state, exact intended edit, and claim before composing execution.

2

2. LOCK ONE EXACT SOURCE PROFILE

Attach one admitted profile and GE-COORD-1.0 receipt; never borrow a missing editor, guide, donor, pegRNA, kit, or numeric condition from another lineage.

조건: One profile ID, source cluster, publication/revision, rights rule, applicability, and exclusion set.
3

3. FREEZE GUIDE AND MODE-SPECIFIC DESIGN

Select one edit mode, preserve guide/PAM orientation and model-versioned scores, and hash the guide, donor or pegRNA artifacts plus explicit specialist handoffs.

4

4. PREDECLARE CONTROLS AND REPLICATES

Assign untreated/parental, delivery/mock, non-targeting, positive/benchmark, and every mode-specific control role before execution.

5

5. EXECUTE ONLY THE TYPED GENE DELIVERY HANDOFF

Use one resolved delivery profile and record actual cargo identities, amount basis and units, model/vessel, reagent or device revision, exposure, recovery, viability, harvest timing, and deviations. ProtocolCore supplies no delivery recipe.

시간: USER REQUIRED — source-defined exposure, recovery, and harvest checkpoints.온도: USER REQUIRED — model and assay-specific handling condition.시약: USER REQUIRED — exact Gene Delivery/Transfection profile ID or UNRESOLVED.
6

6. COLLECT VERSIONED ANALYSIS MATERIAL

Collect every biological replicate at its source-defined time and preserve sample, extraction, amplicon, raw-file, guide/editor/donor/pegRNA, software, and parameter identities.

7

7. CLASSIFY COMPLETE OUTCOMES

Use the selected child chemistry taxonomy and keep intended, bystander, indel, other, unmodified, compound, and unclassified outcomes separate.

8

8. COMPLETE ORTHOGONAL AND RISK REVIEW

Predeclare on-target, orthogonal genotype, structural/dropout, off-target, molecular/function, analytical-sensitivity, and uncertainty dispositions.

9

9. ISSUE A FAIL-CLOSED DISPOSITION

Assign ACCEPT, CONDITIONAL, REPEAT, REJECT, or SPECIALIST REVIEW with critical loss, uncertainty, allowed claim, responsible owner, and immutable evidence links; never collapse discordant evidence into one score.