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BioPlayground

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Bio-Toolkit

Protocol Timer

Step-by-step lab protocol timer

STEPS (0) · 0 min total

Preset을 선택하거나 Add Step으로 단계를 추가하세요

Tool Guide

Definition

Lab protocol timer — A multi-step timer that runs experimental protocols sequentially. Each step ends with an audio alert and auto-advances to the next step.

Purpose

(1) Western blot — automate Block 1h → Primary Ab 2h → Wash 5min×3 → Secondary Ab 1h → Wash 5min×3 (2) ICC/IHC — manage Fixation → Permeabilization → Block → Antibody incubation times (3) Multi-step digestion/ligation (restriction → CIP → ligation) (4) Time-sensitive protocols such as tissue processing and ELISA

How to Use

① Click + Add Step to add a step ② Enter step name and duration (h/m/s) ③ Click Start → first step countdown begins ④ On finish, alarm sounds and auto-advances to the next step Pause/Reset available mid-run. Step reorder and delete supported. Runs even when browser tab is in background.

Examples

Example) Standard Western blot protocol Step 1: Blocking (5% milk/TBST) — 1h Step 2: Primary Ab incubation — 2h Step 3: Wash (TBST) — 5min Step 4: Wash (TBST) — 5min Step 5: Wash (TBST) — 5min Step 6: Secondary Ab incubation — 1h Step 7~9: Wash (TBST) — 5min ×3 → Total 4h 30min auto-sequenced

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🔗 Bio Resources

🔬NCBI PubMed🧪AlphaFold DB🏥ClinicalTrials.gov📄bioRxiv🚀ASGCT🏛️Broad Institute

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