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전체 프로토콜 분야

Gene Delivery / Transfection

출처가 고정된 Gene Delivery / Transfection 시작 프로토콜

공통 identity·실행·결과 계약과 exact source profile 두 개를 제공합니다. 세포, payload, reagent, vessel, scale의 수치를 서로 섞지 않고 CRISPR·Protein Production에는 typed receipt만 전달합니다.

편집 가능한 시작 프로토콜

3 source-bound 시작안

이 프로토콜은 무엇을 하나요?

공통 identity·control·실행·결과 계약 뒤 exact source profile 하나를 선택하고 planned/actual 조건, deviation, delivery, viability와 downstream handoff를 분리해 기록합니다.

지원하는 delivery workflow

  • Common identity, execution, outcome & handoff
  • Suspension HEK293 · PEI-MAX · antibody plasmid
  • MDA-MB-231 · Lipofectamine 3000 · 24-well plasmid

적용 경계

현재 두 exact profile 밖의 세포·payload·reagent·device·format·scale을 추천하거나 자동 보정하지 않으며, viral·in-vivo/LNP·germline·clinical/GMP 실행과 보편 성공 기준은 범위 밖입니다.

PRT-GD-COMMON-001Common identity, execution, outcome & handoff

Gene Delivery identity, source, control, execution, outcome, and typed-handoff foundation

Gene Delivery / Transfection · GD-FAMILY-CONTRACT-1.0

공식 가이드 검토됨
EXACT_SOURCE_PROFILE_REQUIREDMODEL_ROUTE_TOPOLOGY_PAYLOAD_LOCKNO_CROSS_PROFILE_NUMERIC_MIXINGSOURCE_SCALE_ONLY_UNLESS_EXPLICIT_ARITHMETICCONTROL_DISPOSITION_REQUIREDDELIVERY_VIABILITY_AND_OUTCOME_SEPARATETYPED_DOWNSTREAM_HANDOFFNO_UNIVERSAL_ACCEPTANCE_THRESHOLDRESEARCH_USE_ONLY

세포·payload·source profile을 먼저 정확히 맞추세요

현재 실행 가능한 시작안은 suspension HEK293/PEI-MAX antibody DNA와 MDA-MB-231/Lipofectamine 3000 24-well 두 개뿐입니다. 다른 세포·reagent·format은 자동 보정하지 않으며, control과 실제 실행값은 직접 기록해야 합니다.

1

1. LOCK THE BIOLOGICAL MODEL AND PAYLOAD

Record the exact cell identity, source history, passage or adaptation state, topology, payload identity and downstream purpose before selecting a delivery profile.

2

2. SELECT ONE EXACT SOURCE PROFILE

Match route, model, topology, payload, reagent or device, kit or medium, vessel, scale, source revision and locator; any mismatch returns HOLD.

조건: USER REQUIRED — one exact Gene Delivery profile ID or HOLD.
3

3. FREEZE THE SOURCE LEDGER

Preserve each source value separately from derived arithmetic, local qualification inputs and missing fields; never borrow a condition from another profile.

4

4. PREDECLARE READINESS AND CONTROLS

Record source-specific readiness and assign or explicitly omit every control with a reason before execution.

5

5. RECORD PREPARATION AND DELIVERY

Capture planned and actual component identities, amounts, units, order, timing, vessel, exposure and any deviation against the selected source profile.

시간: SOURCE PROFILE REQUIRED
6

6. RECORD RECOVERY AND HANDOFF

Keep recovery conditions and the downstream Protein Production or CRISPR consumer receipt typed and versioned; do not duplicate the downstream recipe.

7

7. SEPARATE DELIVERY AND VIABILITY EVIDENCE

Record delivery readout and viability or morphology as separate observations with sample, control, replicate and time identities.

8

8. RECORD THE DOWNSTREAM BIOLOGICAL OUTCOME

Link expression, editing or another biological outcome without treating it as interchangeable with delivery success.

9

9. ISSUE A FAIL-CLOSED DISPOSITION

Assign READY, CONDITIONAL, REPEAT, REJECT, HOLD or SPECIALIST REVIEW with deviations, uncertainty, allowed claim and immutable evidence links.