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ProtocolCore

Suggested Protocols

探索型MVP
すべてのプロトコル分野

CRISPR / Genome Editing

Source-bound CRISPR / Genome Editing スタータープロトコル

共通計画、SpCas9 knockout、CBE、ABE、HDR、prime editing、結果検証を exact source profile と明示的な境界から構成します。Delivery 条件は Gene Delivery handoff でのみ確定します。

編集可能な開始プロトコル

7 source-bound starter

このプロトコルは何を行いますか？

共通identity contractの後に一つのediting chemistryを選び、source-bound geometry、mode別control、Gene Delivery handoff、非意図product、直交validationを一つのrecordに保存します。

対応するediting workflow

  • • Common identity, controls & handoffs
  • • SpCas9 nuclease knockout
  • • Cytosine base editing
  • • Adenine base editing
  • • HDR precise editing
  • • Prime editing
  • • Outcome validation

適用範囲

異なるeditor・donor・paperの数値を混合せず、delivery recipe、pooled screen、CRISPRi/a、germline・in vivo・clinical実行、普遍的PASS thresholdは対象外です。

PRT-GE-COMMON-001Common identity, controls & handoffs

Genome-editing identity, source, control, delivery, and validation foundation

CRISPR / Genome Editing · GE-COMMON-CONTRACT-1.0

公式ガイド 確認済み
EXACT_SOURCE_PROFILE_REQUIREDGE_COORD_1_REQUIREDNO_CROSS_SOURCE_NUMERIC_MIXINGDELIVERY_HANDOFF_REQUIREDCHEMISTRY_SPECIFIC_OUTCOMESNO_UNIVERSAL_ACCEPTANCE_THRESHOLDRESEARCH_USE_ONLY

実行前に標的・source profile・delivery handoff を確定してください

この MVP starter は標的配列や delivery recipe を生成しません。Reference・transcript・edit intent・exact guide/editor/donor/pegRNA artifact を入力し、実際の Gene Delivery profile と解析根拠に合わせて全条件を確定してください。

1

1. LOCK MODEL, REFERENCE, AND EDIT INTENT

Record model provenance, assembly and patch, contig, 1-based closed coordinate, strand, HGNC ID, transcript accession/version, allele state, exact intended edit, and claim before composing execution.

2

2. LOCK ONE EXACT SOURCE PROFILE

Attach one admitted profile and GE-COORD-1.0 receipt; never borrow a missing editor, guide, donor, pegRNA, kit, or numeric condition from another lineage.

条件: One profile ID, source cluster, publication/revision, rights rule, applicability, and exclusion set.
3

3. FREEZE GUIDE AND MODE-SPECIFIC DESIGN

Select one edit mode, preserve guide/PAM orientation and model-versioned scores, and hash the guide, donor or pegRNA artifacts plus explicit specialist handoffs.

4

4. PREDECLARE CONTROLS AND REPLICATES

Assign untreated/parental, delivery/mock, non-targeting, positive/benchmark, and every mode-specific control role before execution.

5

5. EXECUTE ONLY THE TYPED GENE DELIVERY HANDOFF

Use one resolved delivery profile and record actual cargo identities, amount basis and units, model/vessel, reagent or device revision, exposure, recovery, viability, harvest timing, and deviations. ProtocolCore supplies no delivery recipe.

時間: USER REQUIRED — source-defined exposure, recovery, and harvest checkpoints.温度: USER REQUIRED — model and assay-specific handling condition.試薬: USER REQUIRED — exact Gene Delivery/Transfection profile ID or UNRESOLVED.
6

6. COLLECT VERSIONED ANALYSIS MATERIAL

Collect every biological replicate at its source-defined time and preserve sample, extraction, amplicon, raw-file, guide/editor/donor/pegRNA, software, and parameter identities.

7

7. CLASSIFY COMPLETE OUTCOMES

Use the selected child chemistry taxonomy and keep intended, bystander, indel, other, unmodified, compound, and unclassified outcomes separate.

8

8. COMPLETE ORTHOGONAL AND RISK REVIEW

Predeclare on-target, orthogonal genotype, structural/dropout, off-target, molecular/function, analytical-sensitivity, and uncertainty dispositions.

9

9. ISSUE A FAIL-CLOSED DISPOSITION

Assign ACCEPT, CONDITIONAL, REPEAT, REJECT, or SPECIALIST REVIEW with critical loss, uncertainty, allowed claim, responsible owner, and immutable evidence links; never collapse discordant evidence into one score.