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Suggested Protocols

Exploratory MVP
All protocol fields

Cell Culture Protocol Composer

Define the cell once, then build the culture lifecycle

Do not create one protocol card per cell. Capture human, animal, insect, or other origin in one Cell Record, then compare the official source and current conditions from thawing through banking.

Editable starter protocols

1 lifecycle workflow

What does this protocol do?

It records origin, species, tissue, morphology, growth, source history, and current medium before building the complete culture lifecycle on the same cell record.

Lifecycle coverage

  • • Cell culture lifecycle

Scope boundary

It does not duplicate a protocol for every cell type or combine recipes from different cell banks and manufacturers into a universal condition.

PRT-CC-LIFECYCLE-001Cell culture lifecycle

Cell Culture Lifecycle — select, thaw, seed, maintain, passage, harvest, and bank

Research Cell Culture Lifecycle · PUBLIC-SOURCE-CENSUS-PRT-CC-LIFECYCLE-001-V2

Official guidance reviewed
CELL_RECORD_REQUIRED_BEFORE_THAWORIGIN_SPECIES_TISSUE_AND_CELL_TYPE_REQUIREDCURRENT_MEDIUM_REQUIREDOFFICIAL_SOURCE_BASELINE_REQUIREDSOURCE_AND_CURRENT_CONDITIONS_SEPARATEDADD_REMOVE_REPLACE_DECISION_REQUIRED_FOR_PERSONALIZED_MODEIDENTITY_CONTAMINATION_MORPHOLOGY_GROWTH_QCRESEARCH_USE_ONLY

Add your assay information before use

This draft provides an ordered workflow and rational starting conditions. It intentionally contains no sample primer or probe sequence. Enter your target-specific oligonucleotides and finalize every condition against the actual enzyme, kit, instrument instructions, and assay-validation data.

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0. SELECT AND CHARACTERIZE THE CELL SYSTEM

Create one Cell Record before thawing. Record origin category, species, tissue/organ, cell type, exact name and derivative, donor or host metadata where applicable, disease/normal status, morphology, growth mode, lineage class, engineering, markers/function, source identifiers, passage/PDL, authentication, source-declared biosafety information, and intended use.

Condition: Human, animal, insect, and other origins are metadata branches, not separate protocol families. Do not infer identity, biosafety, or numeric culture conditions from a nickname.
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1. DECLARE THE OFFICIAL SOURCE BASELINE AND CURRENT SYSTEM

Attach the exact current cell-bank, manufacturer, or qualified source and record its revision, locator, and access date. Enter the source baseline and the laboratory condition separately for every lifecycle category, then choose source-aligned or personalized mode.

Reagent: User-entered official source system and current complete culture systemCondition: In personalized mode, explicitly keep, add, remove, replace, or review each category. Never mix manufacturer systems silently.
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2. THAW AND RECOVER

Build the thaw and recovery operation from the selected source: storage transfer, thaw endpoint, aseptic decontamination, dilution or wash/spin, resuspension, recovery vessel and density, incubation, and first recovery observation.

Duration: Enter the source endpoint and local timer checkpoints; no universal thaw time is supplied.Temperature: Enter the exact source and local temperature/atmosphere settings.Condition: Use RCF with rotor radius when a spin is required; do not copy RPM between centrifuges.
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3. COUNT AND SEED

Record the counting method, dilution, total and viable counts, viability criterion, seeding basis, vessel geometry, working volume, mixing/distribution method, and attachment or recovery checkpoint.

Condition: State whether seeding is based on viable cells per area, per volume, per vessel, aggregate, or another source-defined unit.
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4. MAINTAIN, OBSERVE, AND FEED

Define observation cadence and record morphology, attachment or suspension behavior, confluence/density, contamination indicators, medium condition, feeding or exchange, and study-relevant phenotype without relying on calendar alone.

Condition: Origin and growth mode can change the branch substantially; source instructions and observed endpoints remain controlling.
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5. PASSAGE OR SUBCULTURE

Use the source-specific trigger and branch for adherent detachment, suspension split, aggregate handling, or matrix-dependent recovery. Record wash, collection/dissociation endpoint, neutralization, spin, count, reseeding, and passage/PDL update.

Duration: Enter observable endpoints and maximum exposure from the selected source.Condition: A passage ratio is not interchangeable with viable-cell density, and passage number is not interchangeable with cumulative PDL.
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6. HARVEST FOR DOWNSTREAM USE

Define the downstream endpoint first, then record which fractions are retained, collection and wash route, dissociation or lysis, yield/viability/QC, labeling, temperature, time-to-use, and hand-off condition.

Condition: Experimental transfection, differentiation, infection, assay, or manufacturing operations belong in separately bounded protocols.
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7. CRYOPRESERVE AND CLOSE THE BANK RECORD

Record bank tier, harvest state, viable concentration, source-qualified cryomedium, fill volume, cooling method, storage location, vial IDs, freeze date, operator, deviations, and recovery/QC plan.

Temperature: Enter source-qualified cooling and final storage conditions.Condition: A frozen vial is not a qualified bank until identity, traceability, contamination status, recovery, and acceptance records are linked.