TCID50 Calculator
Reed-Muench 또는 Spearman-Kärber 법으로 TCID50 (50% Tissue Culture Infectious Dose)을 산출합니다.
log₁₀ DilPositiveTotal
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TCID50 RESULT (reed-muench)
log₁₀(TCID50)
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TCID50/mL
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≈ PFU/mL (×0.7)
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NOTES
- • Reed-Muench: Cumulative method. Interpolates between dilutions bracketing 50% endpoint via proportional distance.
- • Spearman-Kärber: Requires equal dilution intervals and 100%→0% transition. Uses sum of proportions.
- • TCID50 ≈ 0.7 × PFU (Poisson correction, ATCC guidelines).
- • CPE scoring: record wells showing cytopathic effect as positive.