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Bio-Toolkit

Sequencing Reads Calculator

Calculate required number of reads from target coverage, genome size, and read length. Reverse calculation of coverage-calc.

SEQUENCING REQUIREMENTS

Total Reads

310.00M

Total Data

93.00 Gb

Read Mode

150 × 2 PE

FORMULA

Reads = (Coverage × Genome size) ÷ Read length

For paired-end, effective read length = 2 × read length. Typical coverage: WGS 30×, WES 100×, RNA-seq 20M reads.

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