What is MOI Calculator?

MOI calculator — Compute the volume of virus to add to reach a target Multiplicity Of Infection (viral particles per cell) from cell number, target MOI, and virus titer. Formula: Volume(µL) = (Cell × MOI / Titer) × 1000.

When should I use MOI Calculator?

(1) Lentivirus transduction — MOI 1–10 for stable cell line generation (determine dose before puromycin selection) (2) AAV in vivo / in vitro — MOI 10³–10⁵ range (3) Adenovirus transient expression — MOI 50–200 (4) Standardize dosing for oncolytic, vaccine, and other viral vectors

Can you show me an example of MOI Calculator?

Example 1) HEK293T in 6-well, lentivirus at MOI 10 → Cell: 2×10⁵, MOI: 10, Titer: 1×10⁸ TU/mL → Volume = (2×10⁵ × 10 / 1×10⁸) × 1000 = 20 µL → Add polybrene 8 µg/mL, change medium after 24h Example 2) Primary neurons in 24-well, AAV9 at MOI 10⁵ → Cell: 5×10⁴, MOI: 10⁵, Titer: 1×10¹³ vg/mL → Volume = (5×10⁴ × 10⁵ / 1×10¹³) × 1000 = 0.5 µL

Bio-Toolkit

MOI Calculator

Calculate units-per-target-cell geometry only within an exact profile, lot, and native titer basis

units per target cell = native-basis titer × volume / target cells

The result is units per target cell on the selected native assay basis. GC, VP, TU, PFU, and IFU are never treated as equivalent.

Vector profile ID ID@revisionVector lot IDValueUnitInoculum/delivery volume (mL)Target cell count

Result

INPUT ERROR

Check this

• Check blank values, invalid numbers, row formats, and dispositions. Inputs are never silently changed to zero or exclusion.

The tool does not recommend conditions you did not enter.

Advanced data (optional)

{
  "status": "INPUT_ERROR",
  "error": "SOURCE_PROFILE_REF_FORMAT_INVALID"
}

Tool Guide

Definition

A source-required geometry tool that computes same-basis units per target cell from delivery volume and native titer evidence for one exact vector profile and lot.

Purpose

Lock the lot, assay basis, and profile revision and preserve user-entered delivery geometry in a reproducible receipt. It recommends no universal dose or success condition.

How to Use

Enter an exact profile ID@revision, vector lot, native titer value and unit, delivery volume, and target cell count. Verify that the result basis badge matches the evidence.

Examples

Example: selecting AAV2 GC/mL evidence yields PHYSICAL_GENOMES units per target cell and does not produce TU or PFU.

Bio-Toolkit

MOI Calculator

Calculate units-per-target-cell geometry only within an exact profile, lot, and native titer basis

units per target cell = native-basis titer × volume / target cells

The result is units per target cell on the selected native assay basis. GC, VP, TU, PFU, and IFU are never treated as equivalent.

Vector profile ID ID@revisionVector lot IDValueUnitInoculum/delivery volume (mL)Target cell count

Result

INPUT ERROR

Check this

• Check blank values, invalid numbers, row formats, and dispositions. Inputs are never silently changed to zero or exclusion.

The tool does not recommend conditions you did not enter.

Advanced data (optional)

{
  "status": "INPUT_ERROR",
  "error": "SOURCE_PROFILE_REF_FORMAT_INVALID"
}

Tool Guide

Definition

A source-required geometry tool that computes same-basis units per target cell from delivery volume and native titer evidence for one exact vector profile and lot.

Purpose

Lock the lot, assay basis, and profile revision and preserve user-entered delivery geometry in a reproducible receipt. It recommends no universal dose or success condition.

How to Use

Enter an exact profile ID@revision, vector lot, native titer value and unit, delivery volume, and target cell count. Verify that the result basis badge matches the evidence.

Examples

Example: selecting AAV2 GC/mL evidence yields PHYSICAL_GENOMES units per target cell and does not produce TU or PFU.

BioPlayground

MOI Calculator

Tool Guide

Definition

Purpose

How to Use

Examples

BioPlayground

MOI Calculator

Tool Guide

Definition

Purpose

How to Use

Examples