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Bio-Toolkit
Ligation Calculator
Insert/Vector 몰 비율 및 파이펫팅 레시피 계산
Insert (ng) = Vector (ng) × Ratio × Insert bp / Vector bp
Vector-led
Insert-led
VECTOR
Size (bp)
Amount (ng)
Conc. (ng/µL)
INSERT
Size (bp)
Amount (ng)
Conc. (ng/µL)
MOLAR RATIO (Vector : Insert)
1:1
1:3
1:5
1 :
MASTER MIX SETTINGS
Total Volume (µL)
10
20
Product (optional)
10X Buffer (µL)
Ligase (µL)
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