CRISPR Editing Efficiency Calculator
Calculate indel editing efficiency (%) from T7 Endonuclease I (T7E1) assay gel band densities. Built-in square root correction formula.
Enter densitometry values from gel image analysis (ImageJ, Image Lab, etc.)
EDITING EFFICIENCY
—%
FORMULA
fraction_cleaved = Σ(cleaved bands) / (uncut + Σ(cleaved bands))
% editing = 100 × (1 − √(1 − fraction_cleaved))
The square root correction accounts for the fact that T7E1 cleaves heteroduplexes formed between wild-type and edited alleles (Guschin et al., 2010).
TYPICAL EFFICIENCY RANGES
- • SpCas9 (wild-type): 20–80%
- • High-fidelity Cas9 (eSpCas9, HiFi): 10–60%
- • Cas12a (Cpf1): 10–50%
- • Base editors (CBE/ABE): 10–70% (T7E1 not ideal — use sequencing)