Mismatch-Cleavage Indel Estimator

Estimate indel frequency from uncut, cleaved, and background-corrected mismatch-cleavage bands.

Indel estimate = 100 × (1 − √(1 − corrected cleaved fraction))

Estimate indel rate from mismatch-cleavage bands

Enter uncut and cleaved band intensities. The tool background-corrects them and applies the T7E1-type estimate.

Uncut band intensityCleaved band intensities (comma or space)Background for each band

Estimated indel frequency

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The tool does not recommend conditions you did not enter.

Estimate indel frequency from uncut, cleaved, and background-corrected mismatch-cleavage bands.

Indel estimate = 100 × (1 − √(1 − corrected cleaved fraction))

Enter uncut and cleaved band intensities. The tool background-corrects them and applies the T7E1-type estimate.

Uncut band intensityCleaved band intensities (comma or space)Background for each band

Estimated indel frequency

—

The tool does not recommend conditions you did not enter.