TYPICAL AAV TITERS

• • Crude lysate: 10⁹ – 10¹¹ vg/mL
• • Purified (ultracentrifugation): 10¹² – 10¹³ vg/mL
• • Purified (chromatography): 10¹³ – 10¹⁴ vg/mL
• • Ideal PCR efficiency: 90–110% (slope: −3.6 to −3.1)

ITR CORRECTION NOTE

AAV ITR (Inverted Terminal Repeat) hairpin structures can self-prime during qPCR denaturation, leading to double-counting. When using ITR-targeting primers (e.g. ITR2), enable the ÷2 correction. For transgene-targeting primers, leave unchecked. MspI pre-digestion of DNA prior to qPCR can improve accuracy (Lock et al., 2014).