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探索型MVP
すべてのプロトコル分野

Nucleic Acid Extraction, Purification & QC Foundation

自分のassayに合わせてPCR protocolを完成

実行順序と合理的な開始条件は準備済みです。標的固有のprimer・probeと実際のkit条件を入力し、WordまたはExcelに保存して使用できます。

編集可能な開始プロトコル

4 件

このfoundationは何を行いますか？

試料履歴と対象核酸を記録し、DNAまたはRNAのsource profileを一つ固定した後、実行receiptと複数のQC結果をdownstream適合性判定へ接続します。

対応するfoundation

  • • Sample intake & route lock
  • • DNA extraction & purification
  • • RNA extraction & purification
  • • Orthogonal QC & disposition

適用範囲

異なるkitのlysis・binding・wash・elution条件を混合せず、A260 ratio、RIN、DIN、濃度の一項目だけで普遍的PASSを宣言しません。

PRT-NA-ROUTE-001Sample intake & route lock

Nucleic-acid sample intake and extraction-route lock

Nucleic Acid Extraction, Purification & QC Foundation · PRT-NA-SOURCE-BOUND-PRT-NA-ROUTE-001-V1

公式ガイド 確認済み
SOURCE_PROFILE_REQUIREDSAMPLE_MATRIX_AND_TARGET_LOCKEDNO_CROSS_KIT_RECIPE_MIXINGNO_SINGLE_METRIC_ACCEPTANCEDOWNSTREAM_FIT_REQUIREDRESEARCH_USE_ONLY

使用前に自分のassay情報を入力してください

実行順序と合理的な開始条件を整理した下書きです。サンプルのprimer・probe配列は含めていません。必ず自分の標的配列を入力し、実際の酵素・kit・装置の説明書とassay検証データに合わせて条件を確定してください。

1

1. DEFINE THE SPECIMEN AND TARGET

Record species, matrix, collection device, preservation, storage, freeze-thaw history, input amount, target DNA or RNA class, required fragment-size behavior, and downstream use before selecting a method.

2

2. LOCK ONE EXTRACTION PROFILE

Select one exact manufacturer revision or qualified local SOP that supports the declared matrix, target, scale, and downstream use.

条件: Do not combine lysis, binding, wash, nuclease, or elution conditions from different products.
3

3. DEFINE CONTAMINATION AND FRACTION CONTROLS

Declare extraction blanks, process positives, no-RT or no-template checks, retained fractions, physical segregation, and acceptance ownership before opening samples.

4

4. DEFINE SOURCE-DEPENDENT CAPACITY AND SAFETY

Transcribe the exact input capacity, hazardous reagent combinations, centrifuge or magnetic requirements, and waste handling from the selected source.

試薬: USER REQUIRED — exact kit, lot, buffer preparation, revision, and source locator.
5

5. PREDECLARE THE QC PANEL

Choose fit-for-purpose concentration, purity, integrity or size, target contamination, inhibition, and downstream functional checks; no single ratio is a universal release test.

6

6. LABEL THE HANDOFF CONTRACT

Assign immutable sample, source-profile, extraction-batch, operator, instrument, raw-file, aliquot, and downstream-assay identifiers.