What is Restriction Enzyme Finder?

Restriction enzyme site finder — Searches an input DNA sequence for recognition sites of major type II restriction enzymes (e.g., EcoRI = GAATTC, BamHI = GGATCC, HindIII = AAGCTT) and reports position (bp) and cut count. Searches both forward and reverse complement strands.

When should I use Restriction Enzyme Finder?

(1) Cloning strategy — pick enzymes compatible with both vector MCS and insert (2) Verify restriction digest — compare expected fragment sizes with gel bands (3) Check for unwanted internal cut sites in inserts during subcloning (4) Confirm introduced cut sites after site-directed mutagenesis

Can you show me an example of Restriction Enzyme Finder?

Example) Search pUC19 (2686 bp) for EcoRI + HindIII → EcoRI (GAATTC): 1 cut at position 396 (MCS) → HindIII (AAGCTT): 1 cut at position 447 (MCS) → Double digest yields 51 bp small fragment + 2635 bp backbone → → suitable for cloning (insert between EcoRI/HindIII in MCS)

Bio-Toolkit

Restriction Enzyme Finder

Descriptively scan sequence matches against a finite recognition-motif table

Finite recognition-motif scan only. A match does not establish the current enzyme revision, cut geometry, buffer/activity, methylation response, end compatibility, fragment prediction, or a cloning recommendation. Use an exact source profile for those claims.

DNA SEQUENCE

Tool Guide

Definition

Finite motif-table scanner — reports start positions matching recognition strings in the table. It does not assess enzyme revision, activity, cut geometry, buffer, methylation, or compatibility.

Purpose

(1) Census motif matches in an input sequence (2) Descriptively review candidate locations before selecting an exact enzyme profile (3) Keep source-free output explicitly educational (4) Export positions as TSV for separate validation

How to Use

① Enter a DNA sequence. ② Filter by table label or match count. ③ Review recognition strings and 1-based motif start positions. ④ Validate enzyme revision and cut geometry separately in an exact source profile.

Examples

Example: one GAATTC match produces only its table label, recognition string, start position, and MOTIF_MATCH_ONLY claim. It creates no cut, fragment, MCS, or recommendation claim.

Bio-Toolkit

Restriction Enzyme Finder

Descriptively scan sequence matches against a finite recognition-motif table

DNA SEQUENCE

Tool Guide

Definition

Finite motif-table scanner — reports start positions matching recognition strings in the table. It does not assess enzyme revision, activity, cut geometry, buffer, methylation, or compatibility.

Purpose

How to Use

Examples

Example: one GAATTC match produces only its table label, recognition string, start position, and MOTIF_MATCH_ONLY claim. It creates no cut, fragment, MCS, or recommendation claim.

BioPlayground

Restriction Enzyme Finder

Tool Guide

Definition

Purpose

How to Use

Examples

BioPlayground

Restriction Enzyme Finder

Tool Guide

Definition

Purpose

How to Use

Examples