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Library Concentration Calculator

NGS 라이브러리 농도 변환 (ng/µL ↔ nM)

nM = (ng/µL × 10⁶) / (660 × avg fragment length)

Typical: Illumina ~300bp, Ion Torrent ~200bp

MOLAR CONCENTRATION

0.00nM

REFERENCE

  • • Illumina loading: typically 1.5–2.0 nM (denatured)
  • • Standard pooling: 4 nM recommended
  • • dsDNA constant: 660 g/mol/bp