crispr-cas9-sim — CRISPR-Cas9 시뮬레이션Lab
An education-only toy model for exploring SpCas9/xCas9/Cas12a PAM, cut, and repair concepts with hypothetical weights.
EDUCATIONAL TOY MODEL — candidate scores, self-similarity hits, and repair percentages are hypothetical teaching outputs. Do not use them to select a guide, predict an experiment, compose a protocol, or claim editing efficiency.
SpCas9 = blunt-end DSB · xCas9 = NG broad PAM · Cas12a = staggered DSB
Sliders alter a normalized teaching scenario only. They do not represent donor concentration, cell-cycle measurement, or expected experimental outcome.
Tool Guide
Definition
crispr-cas9-sim is an educational explorer for three PAM architectures, protospacer/cut-site visualization, within-input similarity, and NHEJ/HDR concepts. Scores and repair percentages are toy outputs that do not model locus, cell, or delivery and must not be used for experimental selection or protocol composition.
Purpose
(1) Learn PAM–protospacer relationships (2) Compare Cas9 blunt-end and Cas12a staggered-cut concepts (3) Build intuition that NHEJ and HDR are distinct outcome classes (4) Learn why a real genome-aware design and validation system is required
How to Use
① Enter a short teaching DNA sequence. ② Compare PAM candidates across SpCas9, xCas9, and Cas12a. ③ Visualize a candidate and cut-site concept. ④ Move donor and S/G2 sliders to observe hypothetical repair-weight changes. ⑤ Do not copy any score or percentage into a real protocol, guide choice, or efficiency claim.
Examples
Example: switching between SpCas9 and Cas12a for the same teaching sequence changes PAM orientation and the cut concept. Repair bars are hypothetical teaching weights, not predicted experimental outcomes.