NGS Coverage 계산기 — Read length, read count, target size로부터 평균 sequencing coverage(depth, ×)를 산출하거나, 목표 coverage 달성에 필요한 read 수·data output(Gb)를 역산합니다. 공식: Coverage = (Read length × Number of reads) / Genome size.

(1) WGS — Human 30× = ~90 Gb, germline variant calling 표준 (2) WES — exome 100× = ~10 Gb, somatic mutation 검출 (3) RNA-seq — 20~30M reads/sample (mRNA differential expression) (4) Targeted panel·amplicon seq — 500× 이상 (low-allele frequency variant)

① Application 선택 (WGS / WES / RNA-seq / Targeted / Custom) ② Read length 입력 (예: PE150 = 2×150 = 300 bp 효과적) ③ Number of reads (M reads 또는 reads/sample) ④ Target size (예: Human genome 3 Gb, exome 60 Mb, panel 1 Mb) ⑤ Paired-end / single-end 선택 → 결과: Coverage(×), Total data(Gb), 필요 reads 역산

예 1) Human WGS 30× 목표 → Target: 3 Gb, Read: PE150 (300 bp 효과) → Reads = 3×10⁹ × 30 / 300 = 3×10⁸ = 300 M reads → NovaSeq S4 lane 1개로 충분 예 2) Tumor exome 100× → Target: 60 Mb, PE150 → Reads = 60×10⁶ × 100 / 300 = 20 M reads/sample → NextSeq 한 run에 ~20 sample multiplexing 가능