Buffer Recipe Calculator
Auto-calculate buffer recipes for PBS, Tris-HCl, HEPES, and more from target concentration, pH, and volume.
Most common physiological buffer. Autoclavable.
RECIPE — 1× PBS (pH 7.4) (500 mL)
| Component | Conc. | MW | Amount |
|---|---|---|---|
| NaCl | 137 mM | 58.44 | 4.00 g |
| KCl | 2.7 mM | 74.55 | 0.101 g |
| Na₂HPO₄ | 10 mM | 141.96 | 0.710 g |
| KH₂PO₄ | 1.8 mM | 136.09 | 0.122 g |
BUFFER PROPERTIES
pKa: 7.2
Effective range: 6.2–8.2
Henderson-Hasselbalch:
pH = pKa + log([A⁻]/[HA])