BioPlayground

PC

ProtocolCore

Suggested Protocols

탐색형 MVP
전체 프로토콜 분야

Imaging & Analysis — Fixed-cell Fluorescence Foundation

획득부터 해석 인계까지 lineage가 보존되는 Imaging

Specimen handoff부터 acquisition, immutable raw artifact, processing·segmentation, measurement, replicate summary와 interpretation handoff까지 추적합니다.

편집 가능한 시작 프로토콜

4 source-bound 시작안

이 프로토콜은 무엇을 하나요?

Specimen과 acquisition 설정을 고정하고 raw와 processed artifact를 parent lineage로 연결한 뒤 명시적 background·measurement·replicate·colocalization receipt를 만듭니다.

지원하는 imaging workflow

  • • Common specimen, artifact & replicate contract
  • • Fixed-cell fluorescence acquisition record
  • • Artifact, processing & quantification lineage
  • • Fluorescence spatial-association analysis

적용 경계

이미지 upload·hidden downsample·AI segmentation을 사용하지 않고 widefield와 confocal, field와 biological replicate를 섞지 않으며 universal image/biological PASS나 molecular interaction을 선언하지 않습니다.

PRT-IMG-COMMON-001Common specimen, artifact & replicate contract

Fixed-cell fluorescence specimen, acquisition, artifact and replicate lineage contract

Imaging & Analysis · IMG-FAMILY-CONTRACT-1.0

공식 가이드 검토됨
FIXED_CELL_FLUORESCENCE_FIRST_CHILDIMMUTABLE_RAW_ARTIFACTEXPLICIT_PARENT_AND_PROCESSING_REVISIONWIDEFIELD_AND_CONFOCAL_NOT_COERCEDREPLICATE_LEVELS_NOT_POOLEDEXCLUSIONS_ARE_APPEND_ONLY_LEDGER_ROWSNO_UNIVERSAL_IMAGE_OR_BIOLOGICAL_PASSCOLOCALIZATION_IS_NOT_INTERACTIONNO_SERVER_UPLOAD_OR_HIDDEN_DOWNSAMPLE_OR_AI

이미지 결과보다 lineage를 먼저 고정하세요

Widefield와 confocal 설정을 섞지 않고, 모든 derived artifact를 raw parent에 연결하며, field를 biological replicate 또는 colocalization을 interaction으로 해석하지 않습니다.

1

1. VALIDATE SPECIMEN HANDOFF

Import only minimum specimen identity, staining receipt, preparation revision, groups, controls and unknowns; never copy the staining recipe.

2

2. DECLARE QUESTION AND REPLICATE LEVELS

Predeclare experiment, biological replicate, technical replicate, field and object identities without treating fields as independent biological replicates.

3

3. LOCK MODALITY AND INSTRUMENT

Select widefield, laser-scanning confocal or spinning-disk confocal and record exact instrument, objective, detector and channel identities.

4

4. CAPTURE ACQUISITION SETTINGS

Record pixel calibration, z geometry, bit depth, exposure or dwell, gain, laser power, order, sampling plan and saturation disposition.

5

5. FREEZE RAW ARTIFACT

Create an immutable raw artifact identity with checksum and dimensions. A raw artifact cannot name a processed parent.

6

6. APPEND PROCESSING AND EXCLUSIONS

Every derived artifact names one parent, operation, parameters and software revision; exclusions remain rows with reason and actor.

7

7. SUMMARIZE WITHOUT PSEUDOREPLICATION

Aggregate objects and fields within their explicit hierarchy, then technical replicates within biological replicates; never silently pool levels.

8

8. ISSUE INTERPRETATION HANDOFF

Export measurements, setting mismatches, controls, caveats and unknowns. Do not emit a universal image-quality or biological PASS.