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전체 프로토콜 분야

Cloning & Enzymes

출처와 서열이 고정된 DNA 조립·construct 검증

Restriction-ligation, Type IIS, homology 조립을 섞지 않고 part·junction·expected construct와 관찰 evidence의 lineage를 분리해 추적합니다.

편집 가능한 시작 프로토콜

4 source-bound 시작안

이 프로토콜은 무엇을 하나요?

Construct identity, part sequence, junction geometry와 가정·unknown을 보존하고 transformation과 verification을 typed handoff로 분리합니다.

지원하는 assembly workflow

  • • Common construct, junction, handoff & evidence contract
  • • Restriction-ligation · explicit ratio and enzyme geometry
  • • Type IIS · BsaI-HFv2 ordered overhangs
  • • Homology · NEBuilder HiFi exact junctions

적용 경계

보편 ratio·효소 조건·overhang 품질·변환 recipe를 추천하지 않으며 sequence, digest, colony-PCR evidence를 서로 바꾸거나 construct 전체 PASS를 선언하지 않습니다.

PRT-CL-COMMON-001Common construct, junction, handoff & evidence contract

Cloning identity, ordered-part, junction, handoff and evidence contract

Cloning & Enzymes · CL-FAMILY-CONTRACT-1.0

공식 가이드 검토됨
EXACT_SOURCE_PROFILE_AND_REVISION_REQUIREDNO_DEFAULT_RATIO_OR_RECIPEORDERED_PART_AND_JUNCTION_GRAPHASSUMPTIONS_AND_UNKNOWNS_PRESERVEDTRANSFORMATION_IS_TYPED_HANDOFFVERIFICATION_IS_SEPARATE_CONSUMERNO_GLOBAL_BIOLOGICAL_PASSRESEARCH_USE_ONLY

조립 receipt와 검증 evidence를 분리하세요

검증 ledger는 evidence가 직접 지지하는 축만 비교합니다. Digest나 colony PCR의 일관성이 서열 identity 또는 construct 전체 PASS가 되지 않습니다.

1

1. LOCK CONSTRUCT IDENTITY

Record construct ID, topology, intended use and exact source-profile revision before any method-specific arithmetic or compatibility claim.

2

2. FREEZE ORDERED PARTS

Record every part ID, role, orientation, exact sequence identity, length, checksum and source receipt without filling missing sequence from a motif label.

3

3. DEFINE EVERY JUNCTION

Link upstream and downstream part IDs and preserve strategy, expected sequence, overhang or homology and frame disposition for every junction.

4

4. VALIDATE THE EXACT PROFILE

Check profile ID, revision, source stable ID, applicability and exclusions. Any source, chemistry or revision mismatch returns BLOCKED.

조건: SOURCE REQUIRED — no generic assembly fallback.
5

5. RECORD ASSUMPTIONS AND UNKNOWNS

Keep user assumptions separate from unsupported evidence. Missing editable fields remain INCOMPLETE; unsupported claims remain UNKNOWN.

6

6. ISSUE TRANSFORMATION HANDOFF

Pass only assembly receipt, host requirement, selection identity and unknowns. Do not copy competent-cell amount, recovery time or colony threshold.

7

7. CREATE EXPECTED SNAPSHOT

Freeze construct checksum, expected junctions, critical regions and source references before observing verification evidence.

8

8. COMPARE EVIDENCE PER AXIS

Compare sequence, digest or colony-PCR evidence only on supported axes and preserve discrepancies; never emit a construct-level biological PASS.