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探索型MVP
すべてのプロトコル分野

Imaging & Analysis — Fixed-cell Fluorescence Foundation

Acquisitionからinterpretation handoffまでlineageを保つImaging

Specimen handoffからacquisition、immutable raw artifact、processing・segmentation、measurement、replicate summary、interpretation handoffまで追跡します。

編集可能な開始プロトコル

4 source-bound starter

このプロトコルは何を行いますか？

Specimenとacquisition設定を固定し、rawとprocessed artifactをparent lineageで接続して、明示的なbackground・measurement・replicate・colocalization receiptを作成します。

対応するimaging workflow

  • • Common specimen, artifact & replicate contract
  • • Fixed-cell fluorescence acquisition record
  • • Artifact, processing & quantification lineage
  • • Fluorescence spatial-association analysis

適用範囲

Image upload、hidden downsample、AI segmentationを使用せず、widefieldとconfocal、fieldとbiological replicateを混同せず、普遍的image/biological PASSやmolecular interactionを宣言しません。

PRT-IMG-COMMON-001Common specimen, artifact & replicate contract

Fixed-cell fluorescence specimen, acquisition, artifact and replicate lineage contract

Imaging & Analysis · IMG-FAMILY-CONTRACT-1.0

公式ガイド 確認済み
FIXED_CELL_FLUORESCENCE_FIRST_CHILDIMMUTABLE_RAW_ARTIFACTEXPLICIT_PARENT_AND_PROCESSING_REVISIONWIDEFIELD_AND_CONFOCAL_NOT_COERCEDREPLICATE_LEVELS_NOT_POOLEDEXCLUSIONS_ARE_APPEND_ONLY_LEDGER_ROWSNO_UNIVERSAL_IMAGE_OR_BIOLOGICAL_PASSCOLOCALIZATION_IS_NOT_INTERACTIONNO_SERVER_UPLOAD_OR_HIDDEN_DOWNSAMPLE_OR_AI

画像解釈より先にlineageを固定してください

Widefieldとconfocal設定を混同せず、全derived artifactをraw parentへ接続し、fieldをbiological replicate、colocalizationをinteractionとして扱いません。

1

1. VALIDATE SPECIMEN HANDOFF

Import only minimum specimen identity, staining receipt, preparation revision, groups, controls and unknowns; never copy the staining recipe.

2

2. DECLARE QUESTION AND REPLICATE LEVELS

Predeclare experiment, biological replicate, technical replicate, field and object identities without treating fields as independent biological replicates.

3

3. LOCK MODALITY AND INSTRUMENT

Select widefield, laser-scanning confocal or spinning-disk confocal and record exact instrument, objective, detector and channel identities.

4

4. CAPTURE ACQUISITION SETTINGS

Record pixel calibration, z geometry, bit depth, exposure or dwell, gain, laser power, order, sampling plan and saturation disposition.

5

5. FREEZE RAW ARTIFACT

Create an immutable raw artifact identity with checksum and dimensions. A raw artifact cannot name a processed parent.

6

6. APPEND PROCESSING AND EXCLUSIONS

Every derived artifact names one parent, operation, parameters and software revision; exclusions remain rows with reason and actor.

7

7. SUMMARIZE WITHOUT PSEUDOREPLICATION

Aggregate objects and fields within their explicit hierarchy, then technical replicates within biological replicates; never silently pool levels.

8

8. ISSUE INTERPRETATION HANDOFF

Export measurements, setting mismatches, controls, caveats and unknowns. Do not emit a universal image-quality or biological PASS.