Plate Dilution Planner
Auto-design serial dilution series layouts and pipetting volumes for 96/384-well plates.
Serial dilution results
Enter values to generate a dilution plan.
The arithmetic assumes ideal volume conservation. Confirm pipette working range, dead volume, mixing, adsorption, carryover, vehicle concentration, and last-well discard in the protocol.
Source: NIH/NCATS Assay Guidance Manual — compound dilution plate preparation examples.
Tool Guide
Definition
Plate Dilution Planner — Lay out the start position, direction, concentration steps, and replicates on a 24-, 96-, or 384-well plate, then derive a mathematically consistent transfer volume, diluent volume, and concentration series from the step dilution factor and retained volume.
Purpose
(1) Cross-check serial dilutions for dose-response or ELISA work (2) Prevent mismatches between the concentration series and pipetting volumes (3) Check replicate placement and plate overflow before execution (4) Calculate total source and diluent requirements
How to Use
① Choose the plate, start well, and horizontal or vertical direction. ② Enter concentration-step count, replicate count, starting concentration, and unit. ③ Enter the step dilution factor and volume to retain in every well. ④ Confirm that the calculated transfer volume is within the pipette working range. Each downstream well starts with diluent equal to the retained volume; discard one transfer volume from the last well so retained volumes remain equal.
Examples
Example) 96-well, start A1, 8 horizontal steps, 2 replicates, 1,000 µM, three-fold dilution, retain 100 µL → transfer=50 µL; downstream-well diluent=100 µL → first-well source=150 µL per replicate → total source=300 µL; total diluent=1,400 µL → concentrations=1,000, 333.333, 111.111 … µM Pipetting error, mixing, adsorption, and vehicle effects are not corrected automatically.