BioPlayground

ligand-receptor-sim — 리간드-수용체 신호전달Lab

GPCR (cAMP/IP3-Ca²⁺) / RTK (Ras-MAPK + PI3K-AKT) / nuclear receptors (HRE) / ion channels (GHK) — four receptor cascades integrated (zero external libs, ko/en/ja).

GPCR (cAMP/IP3-Ca²⁺) / RTK (Ras-MAPK + PI3K-AKT) / 핵수용체 (HRE) / 이온채널 (GHK)

Ligand-Receptor Signaling Simulation

Receptor Type

7-TM (seven transmembrane) — Seven transmembrane structure. Ligand binding → conformational change → Gα (GDP→GTP) → 2nd messenger.

Cascade Diagram — GPCR cascade (cAMP-PKA)

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Ligand binding Gα activation (GDP→GTP) AC → cAMP↑ PKA activation CREB phosphorylation → transcription Ligand (e.g., epinephrine) bindsto extracellular pocket of 7-TMreceptor.
Step 1 / 5: Ligand binding

Step 1 — Ligand binding

Ligand (e.g., epinephrine) binds to extracellular pocket of 7-TM receptor.

Ligand-Receptor Examples

Epinephrine→ β2-AR (GPCR Gs)

Effect: Gs-α → AC → cAMP↑ → PKA → CREB. Bronchial smooth muscle relaxation (asthma drug albuterol).

Reference: Pierce 2002 Nat Rev Mol Cell Biol + Lodish Ch.15

Second Messengers

cAMP (cyclic AMP)

Adenylyl Cyclase (AC) → PKA, EPAC

cGMP (cyclic GMP)

Guanylyl Cyclase (GC, NO 활성) → PKG, CNG channels

IP3 (inositol triphosphate)

PLCβ (Gq) / PLCγ (RTK) → IP3 receptor (ER Ca²⁺ release)

DAG (diacylglycerol)

PLCβ / PLCγ → PKC (with Ca²⁺)

Ca²⁺ (calcium)

ER (IP3R, RyR) / 막 (VGCC, SOCE) → Calmodulin → CaMKII, calcineurin

Key terms (hover)

GPCR7TMGαβγcAMPPKACREBRTKSH2RasMAPKPI3KAKTIP3HREnAChRGABA-AcAMP-PKAGHK
Receptor Type (4)
  • GPCR — GPCR (G-protein-coupled receptor) (7-TM (seven transmembrane))
  • RTK — RTK (Receptor Tyrosine Kinase) (Extracellular ligand-binding + intracellular TK)
  • NUCLEAR — Nuclear Receptor (Steroid Hormone Receptor) (DBD (DNA binding) + LBD (ligand binding))
  • IONCH — Ion Channel-Linked Receptor (Ionotropic) (Ligand-gated ion channel (LGIC))

Tool Guide

Definition

ligand-receptor-sim is a free signaling learning tool that integrates four major receptor cascades (GPCR 7TM + Gαsβγ + cAMP/Ca²⁺ + Gs/Gi/Gq branches / RTK dimerisation-phospho-SH2-Ras-MAPK-PI3K-AKT / nuclear receptor HSP90-HRE-coactivator / ion channel nAChR-GABA-A-NMDA-AMPA) as a hierarchical SVG diagram. Four ligand mapping examples (epinephrine GPCR β2 / EGF RTK / cortisol nuclear GR / ACh ion-channel nAChR) + Goldman-Hodgkin-Katz membrane potential + in-house useSvgZoom + useAutoPlay hooks (Lodish Ch.15-16 / Alberts Ch.15 / Pierce 2002 / Lemmon 2010 baseline, zero npm).

Purpose

(1) Undergraduate 4-receptor + cascade static figures → integrated interactive (2) Intuit the 4-way classification (which ligand → which receptor?) — epinephrine / EGF / cortisol / ACh (3) Full cascade steps (Ras-Raf-MEK-ERK / cAMP-PKA-CREB / HRE transcription / GHK membrane potential) (4) In-house svg-zoom + auto-play hook — step-by-step highlight (5) Zero external libs — replace Lodish diagrams with in-house SVG hierarchical layout

How to Use

① Zoom (wheel) + pan (drag) the integrated diagram → 4 receptors in one view ② Pick a ligand (4) → corresponding receptor cascade auto-highlights + auto-play • Epinephrine → β2-AR (GPCR Gs) → Gs-α → AC → cAMP↑ → PKA → CREB • EGF → EGFR (RTK) → dimerisation → autophos → Grb2-SOS → Ras → Raf → MEK → ERK / PI3K → AKT • Cortisol → GR (Type I nuclear receptor) → HSP90 release → dimerisation → nuclear import → GRE → coactivator p300/CBP → transcription • ACh → nAChR (ion channel) → Na⁺/Ca²⁺ channel opens → depolarisation → EPSP → excitation ③ Auto-play ▶ / ⏸ + speed slider (0.5× / 1× / 2×) → step-by-step highlighting ④ Goldman-Hodgkin-Katz equilibrium potential auto-shown (ion-channel scenario) ⑤ Hover 18 academic terms (GPCR / 7TM / Gαβγ / cAMP / PKA / RTK / SH2 / Ras / MAPK / PI3K / AKT / HRE / nAChR / GABA-A, etc.)

Examples

Example 1) Epinephrine → β2-AR → Gs-α → AC → cAMP↑ → PKA → CREB (β-adrenergic) Example 2) EGF → EGFR → dimerisation → Ras-Raf-MEK-ERK + PI3K-AKT (proliferation + survival) Example 3) Cortisol → GR → HSP90 release → GRE → transcription (steroid hormone) Example 4) ACh → nAChR → Na⁺ influx → depolarisation → EPSP (neuromuscular junction)